Neurons in primary cell cultures and the CNS of Drosophila embryos

Top row: Aspects of the embryonic CNS of Drosophila (>>> orientation): staining against the presynaptic marker Synapsin (>>> details), specific neurons labelled via targeted expression of Gal4 (>>> details), central synapses at the ultrastructural level, synaptic transmission in the CNS. Bottom row: All aspects are found in primary cell cultures (serum supplemented Schneider's medium) offering two advantages: 1) visualisation of isolated synapses (bottom left); 2) Cells are deprived of their natural context so that potential signal dependence of developmental mechanisms can be tested.
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Source: top right picture modified from Baines RA, Bate M (1998) Electrophysiological development of central neurons in the Drosophila embryo. J Neurosci 18:4673-4683.